BioSEND can receive many types of biological specimens. Current sample types banked at BioSEND are listed below. If the sample type needed for your project is not listed, please contact us to see if we can add it. We are always eager to expand our collections.

Cerebrospinal Fluid (CSF)

Source
Cerebrospinal Fluid
Standard Collection Tubes
CSF
Conical tubes are utilized as the collection receptacle for the CSF upon extraction. 50.0 ml conical tubes are commonly used by BioSEND for this purpose. LP Trays may also be provided for the collection of CSF. These trays may include: introducer needles, plastic leur lock syringes, and lidocaine.
Standard Processing Procedures
CSF
After CSF collection, the entire specimen is inverted to mix sample and centrifuged within 15 minutes.
CSF is aliquoted into pre-chilled polypropylene 2.0 ml tubes according to the study protocol.
Shipment of Samples to BioSEND
Frozen
CSF aliquots are frozen at -80°C and shipped back to site with dry ice pellets.

Deoxyribonucleic acid (DNA)

Source
Whole Blood, Buffy Coat, and Transferred DNA aliquots
Standard Collection Tubes
Whole Blood and Buffy Coat
The EDTA (Ethylene Diamine Tetra-acetic Acid) tube is utilized in clinical settings to collect whole blood for the purpose of extracting plasma and buffy coat. The EDTA tube in the kit may be plastic or glass.
Transferred DNA
DNA transferred to BioSEND arrives in the required 2.0 ml screw top polypropylene microcentrifuge tubes: DOT Scientific 819-S 2.0 ml screw cap microcentrifuge tubes with O-rings
Standard Processing Procedures
Whole Blood
EDTA tube is inverted 8-10 times to thoroughly mix additive and sample prior to centrifugation.
Shipment of Samples to BioSEND
Frozen
Buffy Coat and transferred DNA aliquots may be shipped back to BioSEND at -80°C temperature on dry ice pellets.
Ambient
Those EDTA collection tubes that do not undergo processing at the location of collection may be sent ambient via next day delivery for processing at BioSEND.

Plasma

Source
Whole Blood
Standard Collection Tubes
Whole Blood
The EDTA (Ethylene Diamine Tetra-acetic Acid) tube is utilized in clinical settings to collect whole blood for the purpose of extracting plasma and buffy coat. The EDTA tubes may be plastic or glass.
Standard Processing Procedures
Whole Blood
The EDTA tube is inverted 8-10 times to thoroughly mix additive and sample prior to centrifugation.
Samples will be centrifuged within 30 minutes of sample collection for the speed and duration dictated by the study protocol. Upon completion of centrifugation, plasma samples are aliquoted for storage and shipment in polypropylene cryovials.
Shipment of Samples to BioSEND
Frozen
Plasma aliquots may be shipped to BioSEND at -80°C temperature packed on dry ice pellets.

Ribonucleic Acid (RNA)

Source
Whole Blood
Standard Collection Tubes
Whole Blood
The PAXgene™ tube is utilized in clinical settings to collect whole blood for the purpose of extracting RNA.
Standard Processing Procedures
Whole Blood
The PAXgene™ tube is never processed at the collection site
The tube is inverted 8-10 times to thoroughly mix additive and sample immediately after blood collection.
If freezing prior to shipment, styrofoam rack use is prohibited. Tube racks made of plastic or wire are approved.
Shipment of Samples to BioSEND
Frozen
PAXgene™ collection tube may be frozen at -80°C and shipped frozen back to BioSEND with dry ice pellets.
Ambient
PAXgene™ collection tube may also be sent ambient via next day delivery.

Serum

Source
Whole Blood
Standard Collection Tubes
Whole Blood
The Serum or Serum Separator (SST) tubes are utilized in clinical settings to collect whole blood for the purpose of extracting serum.
Standard Processing Procedures
Whole Blood
After blood collection, BioSEND standard procedures dictate whole blood processing begin within 60 minutes of specimen collection.
Immediately after blood collection, the serum tube is inverted 8-10 times to thoroughly mix additive and sample.
Blood is then clotted by placing the tube within a tube rack at room temperature for 30 minutes.
Samples will be centrifuged for the speed and duration dictated by the study protocol.
Upon completion of centrifugation, serum samples are aliquoted for storage and shipment in polypropylene cryovials.
Shipment of Samples to BioSEND
Frozen
Serum aliquots are frozen at -80°C and shipped back to BioSEND with dry ice pellets.

Sample Type Not Listed?

Please contact the BioSEND Project Manager, Scott Kaiser, at:
Email
biosend@iu.edu
Local Phone
317-278-0594